G:BOX mini is a compact, multi application imaging system for accurately imaging mini and midi fluorescence and visible gels, multiplex fluorescence westerns, stain free gels and chemiluminescent blots
G:BOX mini 6/9
Compact fluorescent and chemi Western blot imaging system
Need to quantify multiple different fluorescent proteins on one blot and capture images of chemi blots, but your lab is limited on space? Then the G:BOX mini is just the compact imaging powerhouse you’ve been looking for. With stain free, RGB and IR excitation options for Alexa Fluor® 488, 546, 647 and IR dyes, you’re just a click away from brilliant multiplex images from one single Western.
Using the G:BOX mini you’ll also get perfectly exposed images of your chemi labelled proteins without the bother of film. Simply place your blot in the darkroom, click on GeneSys capture software and your imager will do the rest with no fuss and no messy chemicals.
G:BOX mini is a compact, multi-application imaging system combining a unique motor driven stage and cooled high resolution 6 or 9 megapixel camera enabling you to generate accurate optical images, not just digitally enhanced ones. With a G:BOX mini you’ll see separate close chemi and fluorescent bands or spots even on complex gels and know they’re really part of your data.
HI-LED lighting options cover the full spectrum of high intensity blue, green, red and infra-red resulting in faster exposure times and publication quality images.
The system is controlled by GeneSys application driven image capture software and comes complete with unlimited copies of GeneTools analysis software.
Features
Compact fluorescent and chemi Western blot imaging system
- Small footprint
Leaves plenty of room on any lab bench - RGB and IR HI-LED lighting options
Fast quantification of multiple fluorescent proteins without stripping and re-probing - Stain free imaging capability
Capture images of stain free acrylamide gels and many more - High quantum efficiency (QE) camera
Detect picogram or femtogram amounts - Luxury lens (F/0.95 motor driven) with data feedback
Captures the highest quality images - Automatic motor driven stage with automated focus
For real, optical images - White, UV and blue lighting options
Image all gels and blot types on one system - Cleverly designed screen mount option
Easily and securely attach a monitor - GeneSys application driven image capture software
Contains extensive database of dyes and imaging protocols. All you need to know is the type of gel you’re using and GeneSys automatically selects the optimal lighting and filters to produce the perfect image - GeneTools analysis software (unlimited copies)
Analyse data at your own computer
Specifications
Detection with a difference
| SYSTEM | G:BOX mini 6 | G:BOX mini 9 |
| Image resolution (megapixels) | 6 | 9.1 |
| Effective resolution (megapixels) | 18 | 27 |
| A/D | 16 bit | 16 bit |
| Greyscale | 65,536 | 65,536 |
| Quantum efficiency (@425nm) | 73% | 73% |
| Lens (motor driven) | F0.95 | F0.95 |
| Motor driven stage | True optical imaging | True optical imaging |
| Filter wheel (7 position motor driven) | Yes | Yes |
| UV filter | Yes | Yes |
| Use with external PC and printer (not included) | Yes | Yes |
| LIGHTING | ||
| Epi LED white lights | Yes | Yes |
| HI-LED (red, blue, green) | Optional | Optional |
| HI-LED (red, infrared) | Optional | Optional |
| HI-LED (red, blue, green, infrared) | Optional | Optional |
| Visible light converter | Optional | Optional |
| Blue converter screen | Optional | Optional |
| Slim slide-out UV transilluminator (20cm x 20cm) | Optional – 254, 302, 365nm wavelengths | Optional – 254, 302, 365nm wavelengths |
| UltraSlim Blue LED transilluminator Gel size (cm) |
Optional
10 x 12 |
Optional 10 x 12 |
| DIMENSIONS | ||
| Max image area (cm) | 15 x 12 | 15 x 12 |
| Min image area (cm) | 10 x 8 | 10 x 8 |
| W x H x D (cm) | 40 x 64 x 52 | 40 x 64 x 52 |
| Weight (kg) | 40 | 40 |
| Power input (V) | 100-240 | 100-240 |
Accessories
Accessories
Transilluminators
Syngene Slimline transilluminators are available in voltages for 230V 50Hz, 230V 60Hz (-E60), 115V 60Hz (-A), 115V 60Hz (-ACA) and 100V 50/60Hz (-J). Available wavelengths are short wave (254nm, 2020S), medium wave (302nm, 2020M) and long wave (365nm, 2020L).
Blue light converter screens
This comes in size 21 x 26 cm and is used to change harmful UV into blue light. It is made of scratch resistant plastic and is easy to retrofit onto a UV transilluminator.
Visible light converter screens
This come in size 23.5cm x 30cm and is used to change UV light into visible light, ideal for imaging Coomassie Blue and Silver Stained gels.
Filters
The filter wheel takes the 72mm range of filters.
HI-LEDs
The option to add a full spectrum of high intensity blue, green, red and infra-red HI-LEDs that are up to 200 times brighter than standard LEDs, offering faster exposure times and cost-effective imaging for a wide range of fluorescence gel and blot applications.
The excitation ranges of the HI-LEDs (+/- 10nm)
- UV – Peak 365nm
- Blue – Peak 470nm
- Green – Peak 525nm
- Red – Peak 623nm
- Far Red – Peak 658nm
- IR – Peak 740nm
Printer
Any printer with appropriate Windows drivers can be attached to the G:BOX Chemi. We recommend an A6 thermal printer such as Sony or Mitsubishi (P95DW printers sold only for use with Syngene Gel Documentation Products). These USB printers will “plug and play” with the G:BOX Chemi and require minimal set up. They will print hard copies of images in under 5 seconds on long life glossy thermal paper to give photographic quality images.
Thermal paper
Available as Matt or Glossy.
UV tubes
Short wave 8W, 254nm, Medium wave 8W, 302nm, Long wave 8W 365nm available.
IQ/OQ/PQ procedures
More and more laboratories require IQ/OQ/PQ verification these days. Syngene’s IQ/OQ/PQ procedures take the user through each step in the verification of a Syngene system in meeting the demands of the laboratory, regulatory compliances and standard operational procedures (SOP). These simple procedures allow the user to verify that a system meets defined installation, quality and performance specifications.
Installation Qualification (IQ)
This verifies that when a system is unpacked it conforms with all purchasing, shipping and unpacking documents. Once this procedure has been followed the system is then ready for the operational check process.
Operational Qualification (OQ)
This procedure establishes if the system meets all the operation and functionality specifications. An operational test is also included at this stage.
Performance Qualification (PQ)
A performance check will establish if the system meets the specification for the nature of the purpose it was intended for. This is verified by using a simple target to establish if the system can meet performance criteria.
Documentation
Each set of IQ/OQ/PQ documents contains a complete set of procedures and checklists for following the verification steps. Included with each ‘pack’ is a simple calibration device (target image) so that the performance of the system can be checked against the PQ requirements. Each set of documents is unique to a particular system and has to be ordered with the system itself. The document pack has full instructions for use and implementation.
Who does this?
Customer – typically a customer can perform these tests by themselves. Their own internal QC department will be familiar with these processes.
Syngene or its distributors
Trained Syngene personnel or trained distributors can also perform the IQ/OQ/PQ procedures, normally at a nominal charge.