G:BOX Chemi XX6 and XX9 gel doc systems with highest resolution cameras for imaging multiple sample types and sizes, from fluorescence 1D and 2D gels to chemiluminescent blots
G:BOX Chemi XX6/XX9
Walk Away Western Blot Imaging
Your lab’s imaging system shouldn’t control how you detect proteins on Western blots. Chemiluminescence is great if you want sensitive detection of picogram or femtogram amounts, while fluorescence lets you quantify and detect multiple different proteins on one blot. With a G:BOX Chemi XX6/XX9 you can have it all. Powered by Syngene’s revolutionary GeneSys software featuring hundreds of imaging protocols, you’re free to choose chemiluminescence and fluorescence reagents from any manufacturer, putting you in charge of how you detect your proteins.
Featuring an extended darkroom, you can choose between a 6 or 9 mega pixel, cooled, high quantum efficiency camera for unrivaled levels of sensitivity with minimal background interference.
HI-LED lighting options cover the full spectrum of high intensity blue, green, red and infra-red resulting in faster exposure times and publication quality images. An edge lighting option can also be used for 2D gel capture including the use of DIGE gels.
The system is controlled by GeneSys application driven image capture software and comes complete with unlimited copies of GeneTools analysis software.
Gel imaging for fluorescence and chemiluminescence
- High quantum efficiency (QE) camera
For unrivalled levels of sensitivity
- Fast chemiluminescence detection
Walk-away image capture without film
- Motor driven lens and filter wheel
Easy to use, fully automated set up
- IR and RGB HI-LED lighting options
Multiplex fluorescence and protein quantification
- Stain free imaging capability
Capture images of stain free acrylamide gels and many more
- Edge lighting option
2D and multiplex in-gel fluorescence DIGE image capture
- White, UV and blue lighting options
Capture all types of gel and blot images with one system
- Extended darkroom with motor driven stage
Easily image large gels and blots
- GeneSys application driven image capture software
Contains extensive database of dyes and imaging protocols. All you need to know is the type of gel you’re using and GeneSys automatically selects the optimal lighting and filters to produce the perfect image
- GeneTools analysis software (unlimited copies)
Analyse data at your own computer
|G:BOX Chemi XX6||G:BOX Chemi XX9|
|Image resolution (pixels m)||6||9.1|
|Effective resolution (pixels m)||18||27|
|A/D||16 bit||16 bit|
|Quantum efficiency @ 425nm||73%||73%|
|Lens (motor driven)||Motor driven f0.8 or f0.95 with
auto focus and stage zoom
|Motor driven f0.8 or f0.95 with
auto focus and stage zoom
|Filter wheel (7 position motor driven)||Yes||Yes|
|Use with external PC||Yes||Yes|
|Extended with motor driven stage||Yes||Yes|
|Epi LED white lights||Yes||Yes|
|Epi UV 302nm||Optional||Optional|
|Epi red LED module||Optional||Optional|
|Epi blue LED module||Optional||Optional|
|Epi green LED module||Optional||Optional|
|Epi red LED module M series for multiplexing||Optional||Optional|
|Epi green LED module M series for multiplexing||Optional||Optional|
|Epi blue LED module M series for multiplexing||Optional||Optional|
|Epi IR LED module||Optional||Optional|
|IR multiplexing kit (680-800nm)||Optional||Optional|
|Visible light converter 33 x 31cm||Optional||Optional|
|White light pad for visible stains (20 x 14cm)||Optional||Optional|
|UltraBright LED blue light transilluminator 20 x 16cm||Optional||Optional|
|Edge lighting unit 26.5 x 20cm||Optional||Optional|
|Max image area (cm) F0.80 lens||34.5 x 27.6||34.5 x 27.6|
|Min image area (cm) F0.80 lens||15.6 x 12.5||15.6 x 12.5|
|Max image area (cm) F0.95 lens||32.3 x 25.6||32.3 x 25.6|
|Min image area (cm) F0.95 lens||15 x 11.8||15 x 11.8|
|W x H x D (cm)||57 x 99 x 55||57 x 99 x 55|
Syngene offers a range of UV transilluminators suitable for all your imaging needs. Transilluminators are available in 230V 50Hz, 230V 60Hz (-E60), 115V 60Hz (-A), 115V 60Hz (-ACA) and 100V 50/60Hz (-J). Available wavelengths are short wave (254nm, 2020S or 2530S), medium wave (302nm, 2020M or 2530M) and long wave (365nm, 2020L or 2530L).
Blue light transilluminators are often used as an alternative to a UV transilluminator when users wish to use ‘safe dyes’ instead of Ethidium Bromide. A blue light transilluminator is a ‘safe’ light source, in that the user is not exposed to harmful UV radiation and samples are free from photonicking. The UltraBright-LED transilluminator can be used instead of the UV transilluminator in all Syngene G:BOX systems.
UltraBright-LED transilluminator can be used with gels up to 20 x 16cm for use in the G:BOX series. This model uses side mounted 470nm LED arrays for illumination.
UV-blue light converter screens
These come in two sizes 21cm x 26cm and 25cm x 30cm and are used to change harmful UV into blue light. They are made of scratch resistant plastic and are easy to retrofit onto a UV transilluminator.
Visible light converter screens
These come in two sizes 30.5cm x 33cm and 23.5cm x 30cm and are used to change UV light into visible light, ideal for imaging Coomassie Blue and Silver Stained gels.
The Epi UV module can be fitted to any G:BOX Chemi darkroom (those produced after July 2010). The module fits above the standard LED white light module which is standard on all G:BOX Chemi systems. The Epi UV module can have either a 254nm, 365nm or 302nm tube. Either one or two modules can be fitted to each darkroom.
The filter wheel takes the 72mm range of filters.
The option to add a full spectrum of high intensity blue, green, red and infra-red HI-LEDs that are up to 200 times brighter than standard LEDs, offering faster exposure times and cost-effective imaging for a wide range of fluorescence gel and blot applications.
The excitation ranges of the HI-LEDs (+/- 10nm)
- Blue – Peak 470nm
- Green – Peak 525nm
- Red – Peak 623nm
- IR – Peak 740nm
Any printer with appropriate Windows drivers can be attached to the G:BOX Chemi. We recommend an A6 thermal printer such as Sony or Mitsubishi. These USB printers will “plug and play” with the G:BOX Chemi and require minimal set up. They will print hard copies of images in under 5 seconds on long life glossy thermal paper to give photographic quality images.
Available as Matt or Glossy.
Short wave 8W, 254nm, Medium wave 8W, 302nm, Long wave 8W 365nm available.
More and more laboratories require IQ/OQ/PQ verification these days. Syngene’s IQ/OQ/PQ procedures take the user through each step in the verification of a Syngene system in meeting the demands of the laboratory, regulatory compliances and standard operational procedures (SOP). These simple procedures allow the user to verify that a system meets defined installation, quality and performance specifications.
Installation Qualification (IQ)
This verifies that when a system is unpacked it conforms with all purchasing, shipping and unpacking documents. Once this procedure has been followed the system is then ready for the operational check process.
Operational Qualification (OQ)
This procedure establishes if the system meets all the operation and functionality specifications. An operational test is also included at this stage.
Performance Qualification (PQ)
A performance check will establish if the system meets the specification for the nature of the purpose it was intended for. This is verified by using a simple target to establish if the system can meet performance criteria.
Each set of IQ/OQ/PQ documents contains a complete set of procedures and checklists for following the verification steps. Included with each ‘pack’ is a simple calibration device (target image) so that the performance of the system can be checked against the PQ requirements. Each set of documents is unique to a particular system and has to be ordered with the system itself. The document pack has full instructions for use and implementation.
Who does this?
Customer – typically a customer can perform these tests by themselves. Their own internal QC department will be familiar with these processes.
Syngene or its distributors
Trained Syngene personnel or trained distributors can also perform the IQ/OQ/PQ procedures, normally at a nominal charge.
Dr Kevin Feldheim, Laboratory Manager, Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum of Natural History, Chicago, USA
“We’ve had a G:BOX Chemi for over two years and we use it for analysis of DNA fragments to examine relationships between taxa. We compared the G:BOX Chemi with three others from leading manufacturers and chose this system because it makes gel documentation very straightforward and offers the best performance.
We have been very pleased with the G:BOX Chemi and by Syngene’s responsive technical support, so when we needed another gel doc system we knew purchasing a second G:BOX was the right decision”.
Dr Mark K Larson, Assistant Professor, Biology Department, Augustana College, South Dakota, USA
“We use our G:BOX Chemi HR16 regularly for teaching students how to visualize DNA and proteins on gels and proteins on Western blots. The system is simple to work with and the students can use it with very little training. Since the software is so intuitive, it is almost impossible for them to get things wrong!”
“We have also found the real-time, on-line technical support service is excellent. As we’re a bit off the beaten track here, we cannot always wait for an engineer or rep to come out and it’s very reassuring to know Syngene support staff can help us remotely to rapidly resolve any technical questions which arise.”
Professor Darek Gorecki, Director of Research, School of Pharmacy & Biomedical Sciences, University of Portsmouth, Portsmouth, UK
“We know Syngene systems are reliable as we have had a Syngene gel doc for years which has survived being used to its absolute limits. When we needed a second image analyser for imaging Westerns of protein associated with muscular dystrophy, we looked at others but finally stayed loyal to Syngene and installed a G:BOX iChemi XT.”
“This was a great decision because the G:BOX iChemi XT is not as expensive as many that can image fluorescent Westerns, yet the system can detect low levels of proteins using ECL imaging, as well as overlay fluorescent Westerns. This has allowed us to compare different labels directly on the same blot, and more importantly, to have total confidence that the qualitative data on up and down-regulated proteins produced by the G:BOX iChemi XT is accurate.”
Dr Jared Cartwright, Laboratory Head, Technology Facility, University of York, York, UK
“We are a core protein production facility, so we clone and express a wide range of proteins. We chose to install a G:BOX chemiluminescence imaging system in 2008 because of the three we assessed it is the most flexible, has the most user-friendly software and produces a data set that is much simpler to manage for chemiluminescent Westerns than autoradiography.”
“Since then, we’ve seen more and more researchers in the university switching away from using X-ray film for their chemiluminescent blots and over to the G:BOX chemiluminescence imager, which I think shows how easy this system is to use.”
Dr Che-Hsiung Liu, Research Associate, Department of Physiology, Development & Neuroscience, University of Cambridge, Cambridge, UK
“We work with up and down-regulated genes and proteins from fruit fly eyes, which can generate very weak chemiluminescent signal. We stain our DNA with SYBR SafeTM DNA gel stain, protein gels with Coomassie® Blue R and immunoblots with ECL PlusTM, so we have to have an imager that can handle all these applications.”
“After reviewing four imaging systems, we decided to install the G:BOX iChemi XR because it gave us the value of essentially having two systems in one and the GeneTools analysis software is, in my opinion, the best on the market.”
Hannah Cable, Development Scientist, Abcam, Cambridge, UK
“We need quality control methods that are both fast and accurate to make sure we deliver the best antibodies. We used an infrared fluorescence detector for imaging our chemiluminescent Western blots but found it took too long to get the data so in 2007 we installed a G:BOX Chemi XT16 to see if CCD imaging would increase our throughput.
We were delighted to find the G:BOX Chemi XT16 generated accurate results in a fraction of the time and as it costs half the price of an infrared detector it made sense to install another three in 2008. Now we use four G:BOX Chemi XT16 systems to image more than 200 Western blots every week. Obtaining the data is now so much quicker and easier and we are supplying more scientists than ever with high-quality antibodies thanks to our G:BOX Chemi XT16 systems”.
Dr Clare Gallon, Research Associate, Imperial College London, London, UK
“Our G:BOX Chem HR16 has been fantastic. The system is in constant use for analysing both gels and Western blots and does at least 10 runs every day. We have been so impressed with our Syngene image analyser and the excellent technical support team’s can do attitude that we are looking to purchase another Syngene G:BOX specifically for proteomics.”