Agarose gel
Bioluminescence
Camera lens focal lengths
CCD camera
Chemiluminescence
Cloning
Colony counting
Colorimetric blots
DGGE
DIGE 2D gels
DNA
F-Stop
Film
Fluorescence
Fluorophore
Gel documentation
Gel electrophoresis
Gel imaging
GFP plant imaging
In-cell western
In-Gel western
In-vivo imaging
Infra-red imaging
Lens
Membrane
Molecular weight ladder
Multiplexed western blot
Nitrocellulose membranes
PCR
Polyacrylamide gel
Proteins
PVDF membranes
Safe dye imaging
Signal to noise ratio
Stain Free Gels
TGX Stain-free™ gels
Thin Layer chromatography (TLC)
UV shadowing
Western blotting
Fluorescence
Fluorescence occurs when molecules (fluorophores) absorb light in their ground state. Fluorophores do not emit light but when exposed to a light source (excitation by UV, RGB and IR light) their energy levels are elevated to a brief but unstable excited state. As the fluorophore returns to its ground state it emits light at a lower energy which is at a longer wavelength than that of the excited light source.