Definition of Western blotting

Western blotting is a core technique in cell and molecular biology. Western blotting is used to detect the presence of a specific protein in a complex mixture extracted from either cells or tissue.

This technique has three stages; the separation of protein mixtures by size using gel electrophoresis; the efficient transfer of separated proteins to a solid support such as a nitrocellulose or PVDF membrane; and the specific detection of a target protein by appropriately matched antibodies. Once detected, the target protein will be visualized as a band on a blotting membrane on an imaging system.

2D gels

Agarose gel

Bioluminescence

Camera lens focal lengths

CCD camera

Chemiluminescence

Cloning

Colony counting

Colorimetric blots

DGGE

DIGE 2D gels

DNA

F-Stop

Film

Fluorescence

Fluorophore

Gel documentation

Gel electrophoresis

Gel imaging

GFP plant imaging

In-cell western

In-Gel western

In-vivo imaging

Infra-red imaging

Lens

Membrane

Molecular weight ladder

Multiplexed western blot

Nitrocellulose membranes

PCR

Polyacrylamide gel

Proteins

PVDF membranes

Safe dye imaging

Signal to noise ratio

TGX Stain-free™ gels

Thin Layer chromatography (TLC)

UV shadowing

Western blotting