Definition of Multiplexed western blot

Multiplexing fluorescent western blotting allows for the detection and quantification of multiple proteins simultaneously on the same sample. By using highly specific fluorescently labeled antibodies, direct protein abundance comparisons and normalisation against a housekeeping gene can be performed saving you time as you no longer need to strip and re-probe your blot.

2D gels

Agarose gel

Bioluminescence

Camera lens focal lengths

CCD camera

Chemiluminescence