Definition of Membrane
There are two main types of membranes PVDF (polyvinylidene difluoride) and nitrocellulose, both available in different pore sizes and dimensions depending on your application needs.
PVDF membranes are highly hydrophobic and must be pre-wetted with methanol. This type of membrane has a high binding affinity for proteins and nucleic acids and is often used for the following applications - western, southern, northern and dot blots.
Nitrocellulose membranes are popular in protein blotting due to its high-binding affinity and compatibility with a variety of detection methods including chemiluminescence, chromogenic and fluorescence.