Definition of Infra-red imaging

Visible fluorescence can be limited due to high backgrounds from membranes and biological materials. Some proteins autofluoresce strongly in the visible light channels (blue, green and red) also membranes such as PVDF and nitrocellulose can also fluoresce under blue and green light. With Infra-red imaging, membrane and protein autofluorescence is negligible. Commonly used IR secondary antibodies are IRDyes 680 and 800 and DyLight 680 and 800.

2D gels

Agarose gel

Bioluminescence

Camera lens focal lengths

CCD camera

Chemiluminescence

Cloning

Colony counting

Colorimetric blots

DGGE

DIGE 2D gels

DNA

F-Stop

Film

Fluorescence

Fluorophore

Gel documentation

Gel electrophoresis

Gel imaging

GFP plant imaging

In-cell western

In-Gel western

In-vivo imaging

Infra-red imaging

Lens

Membrane

Molecular weight ladder

Multiplexed western blot

Nitrocellulose membranes

PCR

Polyacrylamide gel

Proteins

PVDF membranes

Safe dye imaging

Signal to noise ratio

TGX Stain-free™ gels

Thin Layer chromatography (TLC)

UV shadowing

Western blotting