Agarose gel
Bioluminescence
Camera lens focal lengths
CCD camera
Chemiluminescence
Cloning
Colony counting
Colorimetric blots
DGGE
DIGE 2D gels
DNA
F-Stop
Film
Fluorescence
Fluorophore
Gel documentation
Gel electrophoresis
Gel imaging
GFP plant imaging
In-cell western
In-Gel western
In-vivo imaging
Infra-red imaging
Lens
Membrane
Molecular weight ladder
Multiplexed western blot
Nitrocellulose membranes
PCR
Polyacrylamide gel
Proteins
PVDF membranes
Safe dye imaging
Signal to noise ratio
Stain Free Gels
TGX Stain-free™ gels
Thin Layer chromatography (TLC)
UV shadowing
Western blotting
Glossary definition of
Definition of Infra-red imaging
Visible fluorescence can be limited due to high backgrounds from membranes and biological materials. Some proteins autofluoresce strongly in the visible light channels (blue, green and red) also membranes such as PVDF and nitrocellulose can also fluoresce under blue and green light. With Infra-red imaging, membrane and protein autofluorescence is negligible. Commonly used IR secondary antibodies are IRDyes 680 and 800 and DyLight 680 and 800.