Definition of Infra-red imaging
Visible fluorescence can be limited due to high backgrounds from membranes and biological materials. Some proteins autofluoresce strongly in the visible light channels (blue, green and red) also membranes such as PVDF and nitrocellulose can also fluoresce under blue and green light. With Infra-red imaging, membrane and protein autofluorescence is negligible. Commonly used IR secondary antibodies are IRDyes 680 and 800 and DyLight 680 and 800.