Agarose gel
Bioluminescence
Camera lens focal lengths
CCD camera
Chemiluminescence
Cloning
Colony counting
Colorimetric blots
DGGE
DIGE 2D gels
DNA
F-Stop
Film
Fluorescence
Fluorophore
Gel documentation
Gel electrophoresis
Gel imaging
GFP plant imaging
In-cell western
In-Gel western
In-vivo imaging
Infra-red imaging
Lens
Membrane
Molecular weight ladder
Multiplexed western blot
Nitrocellulose membranes
PCR
Polyacrylamide gel
Proteins
PVDF membranes
Safe dye imaging
Signal to noise ratio
Stain Free Gels
TGX Stain-free™ gels
Thin Layer chromatography (TLC)
UV shadowing
Western blotting
Glossary definition of
Definition of Fluorescence
Fluorescence occurs when molecules (fluorophores) absorb light in their ground state. Fluorophores do not emit light but when exposed to a light source (excitation by UV, RGB and IR light) their energy levels are elevated to a brief but unstable excited state. As the fluorophore returns to its ground state it emits light at a lower energy which is at a longer wavelength than that of the excited light source.