Definition of Fluorescence

Fluorescence occurs when molecules (fluorophores) absorb light in their ground state. Fluorophores do not emit light but when exposed to a light source (excitation by UV, RGB and IR light) their energy levels are elevated to a brief but unstable excited state. As the fluorophore returns to its ground state it emits light at a lower energy which is at a longer wavelength than that of the excited light source.

2D gels

Agarose gel

Bioluminescence

Camera lens focal lengths

CCD camera

Chemiluminescence

Cloning

Colony counting

Colorimetric blots

DGGE

DIGE 2D gels

DNA

F-Stop

Film

Fluorescence

Fluorophore

Gel documentation

Gel electrophoresis

Gel imaging

GFP plant imaging

In-cell western

In-Gel western

In-vivo imaging

Infra-red imaging

Lens

Membrane

Molecular weight ladder

Multiplexed western blot

Nitrocellulose membranes

PCR

Polyacrylamide gel

Proteins

PVDF membranes

Safe dye imaging

Signal to noise ratio

TGX Stain-free™ gels

Thin Layer chromatography (TLC)

UV shadowing

Western blotting