Definition of DIGE 2D gels

DIGE is a 2D gel that uses direct labeling of proteins with fluorescent dyes (known as CyDyes: Cy2, Cy3 and Cy5) making it easy to multiplex different protein samples within the same 2D gel. This permits the inclusion of an internal standard within each gel, limiting the experimental variation and ensures accurate intra-and inters gel matching.

2D gels

Agarose gel

Bioluminescence

Camera lens focal lengths

CCD camera

Chemiluminescence

Cloning

Colony counting

Colorimetric blots

DGGE

DIGE 2D gels

DNA

F-Stop

Film

Fluorescence

Fluorophore

Gel documentation

Gel electrophoresis

Gel imaging

GFP plant imaging

In-cell western

In-Gel western

In-vivo imaging

Infra-red imaging

Lens

Membrane

Molecular weight ladder

Multiplexed western blot

Nitrocellulose membranes

PCR

Polyacrylamide gel

Proteins

PVDF membranes

Safe dye imaging

Signal to noise ratio

TGX Stain-free™ gels

Thin Layer chromatography (TLC)

UV shadowing

Western blotting