Definition of DGGE

Denaturing gradient gel electrophoresis (DGGE) is based on finger printing methodology. The gel is subjected to a constant heat of ~600C and increasing concentration of denaturing chemicals are used to force DNA molecules to un-fold (melt) which are also known as melting domains. Any variation of DNA sequences within these domains will result in different melting temperatures causing different sequences to migrate at different positions in the gel.

2D gels

Agarose gel

Bioluminescence

Camera lens focal lengths

CCD camera

Chemiluminescence

Cloning

Colony counting

Colorimetric blots

DGGE

DIGE 2D gels

DNA

F-Stop

Film

Fluorescence

Fluorophore

Gel documentation

Gel electrophoresis

Gel imaging

GFP plant imaging

In-cell western

In-Gel western

In-vivo imaging

Infra-red imaging

Lens

Membrane

Molecular weight ladder

Multiplexed western blot

Nitrocellulose membranes

PCR

Polyacrylamide gel

Proteins

PVDF membranes

Safe dye imaging

Signal to noise ratio

TGX Stain-free™ gels

Thin Layer chromatography (TLC)

UV shadowing

Western blotting