Definition of Colorimetric blots

After transfer and before proceeding with the western blot, total protein on the membrane is often stained with a dye, such as Ponceau S or amido black 10B to check transfer efficiency.

Most dyes interfere with antibody binding and detection therefore, a protein stain that is easily removable is ideal such as Ponceau S.

2D gels

Agarose gel


Camera lens focal lengths

CCD camera