To visualise colourless spots or bands on a TLC (Thin Layer Chromatography) plate, you can either use a plate with a fluorescent indicator or view it under UV light or you can spray reagents onto the samples to colour them so that spots are visible in white light. You can detect the spots using a colour CCD-based TLC plate imager equipped with overhead UV and white lighting.
Using its extensive imaging expertise, Syngene has developed the Chromascan systems for automated imaging of TLC plates and ChromaTools software for analysis, so you’ll find the right TLC plate reader to meet your budget and the level of analysis your TLC applications demand.
With a Chromascan system you can perform TLC imaging on plates including those coated with silica gel and aluminium oxide and you can spray on reagents such as ninhydrin and Rhodamine (depending on your sample) to colour your sample spots.
To automate the analysis of TLC plates you can capture an image of the plate using Chromascan software. Then you can analyse the image with ChromaTools software which separates multiple overlapping spots, marking true spots and bands to automatically generate Rf data or chromatogram peaks. This will provide quantitative information such as the percentage total activity for each peak, thus reducing the time and effort you spend performing manual calculations.
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