For high performance imaging, the new generation of G:BOX Chemi systems provide the user with an extended range of applications covering fluorescence and chemiluminescence. All these systems now utilise Syngene’s groundbreaking GeneSys automatic control software. Driven from a database containing hundreds of capture protocols, full manual control is available should you wish to use it.
The G:BOX Chemi series features cameras with resolutions of 4m, 6m or 9m pixels. All have cooling which reduces the small amount of electronically generated noise to produce outstanding images with minimal background interference. These new generation cameras also have higher quantum efficiencies (QE) than most, helping the system to achieve outstanding levels of sensitivity.
Whether using the system for standard fluorescence applications such as DNA, or for chemiluminescence imaging, the G:BOX Chemi will give you high quality results. In addition, by using the optional LED modules users can work with a unique automated multiplexed function capable of using up to 5 channels. Other LED modules can be used to turn the G:BOX Chemi into a highly sensitive IR capture system. An edge lighting option can also be used for 2D gel capture including the use of DIGE gels.
The stylish design with modular construction includes motor-driven lenses, filter wheels with the option of including a range of lighting choices for both Epi and transillumination applications.
The system comes complete with unlimited copies of GeneTools analysis software.
|G:BOX F3||G:BOX Chemi XRQ||G:BOX Chemi XT4||G:BOX Chemi XX6||G:BOX Chemi XX9|
|Image resolution (pixels m)||3.8||4||4.2||6||9|
|Effective resolution (pixels m)||15.3||16||16.76||18||27|
|A/D||12/16 bit||16 bit||16 bit||16 bit||16 bit|
|Quantum efficiency @ 425nm||N/A||73%||53%||73%||73%|
|Cooling - regulated (degrees)
|Lens (motor driven)||f1.2||f1.2 with feed back||f0.95 with auto focus||f1.4 with auto focus||f1.4 with auto focus|
|Filter wheel (7 position motor driven)||Yes||Yes||Yes||Yes||Yes|
|Use with external PC||Yes||Yes||Yes||Yes||Yes|
|Extended with motor driven stage||Yes||Yes||Yes|
|Epi LED white lights||Yes||Yes||Yes||Yes||Yes|
|Epi UV 302nm||Optional||Optional||Optional||Optional||Optional|
|Epi red LED module||Optional||Optional||Optional||Optional|
|Epi blue LED module||Optional||Optional||Optional||Optional|
|Epi green LED module||Optional||Optional||Optional||Optional|
|Epi red LED module M series for multiplexing||Optional||Optional||Optional||Optional|
|Epi green LED module M series for multiplexing||Optional||Optional||Optional||Optional|
|Epi blue LED module M series for multiplexing||Optional||Optional||Optional||Optional|
|Epi IR LED module||Optional||Optional||Optional||Optional|
|IR multiplexing kit (680-800nm)||Optional||Optional||Optional||Optional|
|Visible light converter 33 x 31cm||Optional||Optional||Optional||Optional||Optional|
|White light pad for visible stains (20 x 14 or 20 x 30cm)||Optional||Optional||Optional||Optional||Optional|
|UltraBright LED blue light transilluminator 20 x 16cm||Optional||Optional||Optional||Optional||Optional|
|Edge lighting unit 26.5 x 20cm||Optional||Optional||Optional|
|Max image area (cm)||25.5 x 21||30.5 x 22.7||19 x 19||22.1 x 17.6||22.1 x 17.6|
|Min image area (cm)||4.5 x 3.8||5.5 x 4||9 x 9||10 x 8||10 x 8|
|W x H x D (cm)||57 x 84 x 45||57 x 84 x 45||57 x 99 x 55||57 x 99 x 55||57 x 99 x 55|
The biggest difference between a Syngene system and any other is in the way the system is controlled. At the heart of all Syngene systems is GeneSys which is an application driven control software. With G:BOX you can use GeneSys in either Automatic or Manual mode.
It is assumed that the user will know exactly what their application is and how they have prepared their gel or blot. For Automatic capture the user simply selects either Gels or Blots and from the drop down list select the sample type or recall this information from a saved protocol and the system takes over the rest. Behind GeneSys is an extensive database which contains data relating to a very wide range of applications, e.g. fluorescence, chemiluminescence and chemifluorescence. Once the G:BOX has been told what sample to expect then GeneSys decides what hardware configuration is best and sets the system ready for image capture. Things such as camera control, exposure time, sensitivity setting, lighting requirements, lens control, filter selection- all of this is taken care of by GeneSys. The user just has to click the ’capture’ button and wait for the perfect image to appear.
GeneSys image aquisition software
From the opening screen the user can select the mode of operation – Auto or Manual. It is also possible to access the image browser and enter the edit screen to work on previously stored images. Previously stored saved protocols are shown on the left hand side of the home screen.
GeneSys image aquisition software
For automatic capture of Gels or Blots the user simply selects the ‘sample format’ from the pop up menu and the sample size from drop down menu. The software has been designed for use with a touch screen.
GeneSys image aquisition software
Once the application type has been selected, GeneSys then offers the user a range of dyes from its database. The user then simply selects the dye. It is also possible to undertake multiplex applications in which case the user would select each dye from the available list. The database behind GeneSys contains over 250 different dyes and stains and is updated on a regular basis as more options come onto the market.
Once the dye has been selected then the system will automatically configure itself to capture the best possible image of the sample. The lighting, filter and camera exposure settings are all automatically adjusted for the sample type. The captured image is displayed in the main screen with small thumbnail images of the current and previous images (either opened or captured) shown in an image ‘pool’. Note the icons at the foot of the screen which enable previous pages to be selected.
The fully automatic functions of GeneSys allow even the most difficult or complicated gels and blots to be imaged. For example with Chemiluminescence the system uses its intelligence to know the output profile of most of the major substrates. It can then decide the exposure setting best suited to a particular substrate. Then during the image capture process it continually monitors the output from the blot until the best image is produced.
Another example of the power of GeneSys is when working with multiplex blots. Once the application information is selected by the user GeneSys will make separate exposures using the correct filter, lighting and camera setting for each dye. The final multiplex image will be displayed.
On the image edit page the user can enhance the image, add annotation, save or print.
Not everyone wants their system to take automatic control. Therefore, there is the ability for the user to take complete control of every function of the system by using the manual mode. GeneSys has been designed using the most advanced software tools available. Primarily it has been designed for use with a ‘touch’ panel PC and to take advantage of the new functions afforded by Windows 7. Selection of all options using the simple interface is easy and intuitive.
G:BOX Chemi XRQ
The XRQ is a superb flexible system suitable for all your fluorescence imaging needs and also for low light level chemiluminescence from blots and plates.
The XRQ has 16 million effective pixels with a standard image output of 4 million pixels. The sensor is one of the new generation sensors which has exceptional Quantum Efficiency (QE), meaning that the camera is very sensitive indeed. This makes it perfect for low light applications like Western blot chemiluminescence samples. With the XRQ you are guaranteed superb images of gels, blots and plates. The camera has a 16 bit maximum dynamic range.
Naturally the camera is cooled and has very low noise so imaging chemiluminescent samples is easy. Using GeneSys software makes capture of any sample a simple, automatic process. Exposure times of many hours are possible and always result in clean, crisp images.
The G:BOX XRQ is a highly versatile system capable of imaging all routine samples, EtBr and safe alternatives, SYBR, coomassie plus chemi blots, colony plates and many fluorescent applications including red, green and blue excitation.
The XRQ LED comes with LED gantry rack and epi white LED lighting, optional UV or blue transilluminator and visible light converter. It is simple and cost effective to fit epi UV, blue, green and red lighting either at time of purchase or later. There is a seven position motor driven filter wheel. The entire system is fully motor driven with lens feedback, filter wheel, lighting and exposure time, all controlled, regulated and optimised through the powerful GeneSys acquisition software.
The XRQ offers high performance at an affordable cost, it is suitable for a lab using many sample types requiring versatility whilst retaining simplicity.
G:BOX Chemi XT4
The XT4 is the first of our high end systems, giving excellent high resolution images every time. It is suitable for all your fluorescence imaging needs and also low light level chemiluminescence from blots and plates. It is capable of imaging all routine samples, EtBr and safe alternatives, SYBR, coomassie, chemi blots, colony plates and many fluorescent applications including red, green, blue and IR excitation.
The 16 bit camera has 4.2m pixels of resolution. With large pixels there is rarely any need to select binning even when looking at very low light levels. The fully motor driven sample stage optimises the distance between camera and sample each time giving maximum resolution and sensitivity.
The XT4 comes with LED gantry rack and epi white LED lighting, optional UV or blue transilluminator and visible light converter. It is simple and cost effective to fit epi UV, blue, green, red or IR lighting either at time of purchase or later. There is a seven position motor driven filter wheel. The entire system is fully motor driven with lens feedback, filter wheel, lighting, sample stage and exposure time, all controlled, regulated and optimised through the powerful GeneSys acquisition software.
The XT4 has enough resolution to cope with 2D gels, even some microscope slides can be imaged in detail with this flexible system. For DIGE samples an edge illumination unit can be fitted to give the best results.
An essential component in any lab with multiple sample types and a requirement for high quality images every time.
G:BOX Chemi XX6 and XX9
The XX6 and XX9 are top of the range, high resolution imaging systems, suitable for the most demanding users and applications. They are suitable for all your fluorescence imaging needs and also low light level chemiluminescence from blots and plates. They are capable of imaging all routine samples, EtBr and safe alternatives, SYBR, coomassie, chemi blots, colony plates and many fluorescent applications including red, green, blue and IR excitation.
The XX6 has a resolution of 6.0m pixels, whilst the XX9 has a resolution of 9.0m pixels. Both of them are 16 bit cameras. They both offer very flexible binning options leading to extremely fast capture times and feature a fully motor driven sample stage which optimises the distance between camera and sample each time resulting in maximum resolution and sensitivity.
Both come with LED gantry rack and epi white LED lighting, optional UV or blue transilluminator and visible light converter.. It is simple and cost effective to fit epi UV, blue, green, red or IR lighting either at time of purchase or later. Both systems are fully motor driven with lens, a 7 position filter wheel, lighting, sample stage and exposure time, all controlled, regulated and optimised through the powerful GeneSys acquisition software.
The XX6 and XX9 have enough resolution to cope with even the smallest 2D gels. Also some microscope slides can be imaged in great detail with these flexible systems. For DIGE samples an edge illumination unit can be fitted to give the best results.
The high specification XX6 or XX9 systems are an essential component for the lab with multiple sample types and where only the very highest quality images are acceptable.
Blue light transilluminators are often used as an alternative to a UV transilluminator when users wish to use ‘safe dyes’ instead of Ethidium Bromide. A blue light transilluminator is a ‘safe’ light source, in that the user is not exposed to harmful UV radiation and samples are free from photonicking. The UltraBright-LED transilluminator can be used instead of the UV transilluminator in all Syngene G:BOX systems.
UltraBright-LED transilluminator can be used with gels up to 20 x 16cm for use in the G:BOX series. This model uses side mounted 470nm LED arrays for illumination.
Syngene offers a range of UV transilluminators suitable for all your imaging needs.
The Epi UV module can be fitted to any G:BOX Chemi darkroom (those produced after July 2010). The module fits above the standard LED white light module which is standard on all G:BOX Chemi systems. The Epi UV module can have either a 254nm, 365nm or 302nm tube. Either one or two modules can be fitted to each darkroom.
The filter wheel takes the 58mm range of filters.
For a more versatile G:BOX Chemi system, LED light modules for red, green and blue light can be fitted to an optional lighting gantry.
With this new lighting system the user can select which wavelength of light they require for any specific application. The lighting modules are single wavelength and contain LEDs and a dedicated filter. Each module is "plug and play". Once plugged into the gantry the module is electronically registered by the G:BOX Chemi which recognises the wavelength and type of filter being used.
Up to 4 modules can be plugged into each side of the darkroom. It is possible to fit identical modules to increase intensity or to use a number of modules with different wavelengths. Modules are normally fitted in matched pairs, one either side.
There are 8 different LED modules to choose from:
Module Wavelength Filter type Applications
Blue 465 Cut off General
Blue (m) 465 Band pass Close range multiplex apps
Green 520 Cut off General
Green (m) 520 Band pass Close range multiplex apps
Red 635 Cut off General
Red (m) 635 Band pass Close range multiplex apps
IR 740 Cut off General
IR (m) 740 Band pass Close range multiplex apps
Please refer to the Syngene database for the comprehensive list of dyes which can be used with the LED light modules.
Any printer with appropriate Windows drivers can be attached to the G:BOX Chemi. We recommend an A6 thermal printer such as Sony or Mitsubishi. These USB printers will "plug and play" with the G:BOX Chemi and require minimal set up. They will print hard copies of images in under 5 seconds on long life glossy thermal paper to give photographic quality images.
More and more laboratories require IQ/OQ/PQ verification these days. Syngene’s IQ/OQ/PQ procedures take the user through each step in the verification of a Syngene system in meeting the demands of the laboratory, regulatory compliances and standard operational procedures (SOP). These simple procedures allow the user to verify that a system meets defined installation, quality and performance specifications.
Installation Qualification (IQ)
This verifies that when a system is unpacked it conforms with all purchasing, shipping and unpacking documents. Once this procedure has been followed the system is then ready for the operational check process.
Operational Qualification (OQ)
This procedure establishes if the system meets all the operation and functionality specifications. An operational test is also included at this stage.
Performance Qualification (PQ)
A performance check will establish if the system meets the specification for the nature of the purpose it was intended for. This is verified by using a simple target to establish if the system can meet performance criteria.
Each set of IQ/OQ/PQ documents contains a complete set of procedures and checklists for following the verification steps. Included with each ‘pack’ is a simple calibration device (target image) so that the performance of the system can be checked against the PQ requirements. Each set of documents is unique to a particular system and has to be ordered with the system itself. The document pack has full instructions for use and implementation.
Who does this?
Customer - typically a customer can perform these tests by themselves. Their own internal QC department will be familiar with these processes.
Syngene or its distributors
Trained Syngene personnel or trained distributors can also perform the IQ/OQ/PQ procedures, normally at a nominal charge.
“We’ve had a G:BOX Chemi for over two years and we use it for analysis of DNA fragments to examine relationships between taxa. We compared the G:BOX Chemi with three others from leading manufacturers and chose this system because it makes gel documentation very straightforward and offers the best performance.
We have been very pleased with the G:BOX Chemi and by Syngene’s responsive technical support, so when we needed another gel doc system we knew purchasing a second G:BOX was the right decision”.
“We use our G:BOX Chemi HR16 regularly for teaching students how to visualize DNA and proteins on gels and proteins on Western blots. The system is simple to work with and the students can use it with very little training. Since the software is so intuitive, it is almost impossible for them to get things wrong!”
“We have also found the real-time, on-line technical support service is excellent. As we’re a bit off the beaten track here, we cannot always wait for an engineer or rep to come out and it’s very reassuring to know Syngene support staff can help us remotely to rapidly resolve any technical questions which arise.”
“We know Syngene systems are reliable as we have had a Syngene gel doc for years which has survived being used to its absolute limits. When we needed a second image analyser for imaging Westerns of protein associated with muscular dystrophy, we looked at others but finally stayed loyal to Syngene and installed a G:BOX iChemi XT.”
“This was a great decision because the G:BOX iChemi XT is not as expensive as many that can image fluorescent Westerns, yet the system can detect low levels of proteins using ECL imaging, as well as overlay fluorescent Westerns. This has allowed us to compare different labels directly on the same blot, and more importantly, to have total confidence that the qualitative data on up and down-regulated proteins produced by the G:BOX iChemi XT is accurate.”
“We are a core protein production facility, so we clone and express a wide range of proteins. We chose to install a G:BOX chemiluminescence imaging system in 2008 because of the three we assessed it is the most flexible, has the most user-friendly software and produces a data set that is much simpler to manage for chemiluminescent Westerns than autoradiography.”
“Since then, we’ve seen more and more researchers in the university switching away from using X-ray film for their chemiluminescent blots and over to the G:BOX chemiluminescence imager, which I think shows how easy this system is to use.”
“We work with up and down-regulated genes and proteins from fruit fly eyes, which can generate very weak chemiluminescent signal. We stain our DNA with SYBR SafeTM DNA gel stain, protein gels with Coomassie® Blue R and immunoblots with ECL PlusTM, so we have to have an imager that can handle all these applications.”
“After reviewing four imaging systems, we decided to install the G:BOX iChemi XR because it gave us the value of essentially having two systems in one and the GeneTools analysis software is, in my opinion, the best on the market.”
“We need quality control methods that are both fast and accurate to make sure we deliver the best antibodies. We used an infrared fluorescence detector for imaging our chemiluminescent Western blots but found it took too long to get the data so in 2007 we installed a G:BOX Chemi XT16 to see if CCD imaging would increase our throughput.
We were delighted to find the G:BOX Chemi XT16 generated accurate results in a fraction of the time and as it costs half the price of an infrared detector it made sense to install another three in 2008. Now we use four G:BOX Chemi XT16 systems to image more than 200 Western blots every week. Obtaining the data is now so much quicker and easier and we are supplying more scientists than ever with high-quality antibodies thanks to our G:BOX Chemi XT16 systems”.
“Our G:BOX Chem HR16 has been fantastic. The system is in constant use for analysing both gels and Western blots and does at least 10 runs every day. We have been so impressed with our Syngene image analyser and the excellent technical support team’s can do attitude that we are looking to purchase another Syngene G:BOX specifically for proteomics.”
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