What to consider when purchasing a Western blot and Gel imaging system

/What to consider when purchasing a Western blot and Gel imaging system
What to consider when purchasing a Western blot and Gel imaging system2018-07-03T16:58:19+00:00

Whether you are looking to move out of the darkroom and invest in your first digital imaging system or if you are looking to replace an old system, it is important to purchase the right equipment that will adapt with your research.

Before making a decision consider the following points to help you select the perfect imaging system for your laboratory.

1

Decode specifications

Users commonly look at the sensor resolution of the camera when evaluating gel documentation imaging systems. However, it is just not all about resolution. Dynamic range, sensitivity and camera lens all affect how good a camera is. The higher the resolution the camera is, will enable you to see fine detail in your images making it easier to distinguish close bands on your gel or blot.

A wide dynamic range allows you to see both your strong and weak bands on the same blot. If your strong bands saturate you will be unable to quantify your data therefore make sure you pick an imaging system with a good dynamic range.

When looking at lenses you need to consider the F stop. Smaller F stops are especially important for chemiluminescence as smaller F stops let more light into the sensor and can drastically reduce exposure times.

2

Versatile

Short on bench space? Then maximise your space and budget with an imaging system that is versatile and capable of imaging a wide range of applications in one system.

Choose a system that is upgradeable with multiple light sources compatible with the commonly commercially available dyes and stains. Ensure you have selected the right light source for your application. Use Hi LEDs for multiplexing RGB and IR fluorescent blots and gels, UV transilluminator for DNA/RNA gels, visible light converter screen for Coomassie blue and silver stained protein gels and blue converter screen for imaging ‘safe’ dyes.

3

Ease of use

Whichever system you choose you want a system that is intuitive and can be used by everyone without training. Getting publication quality images should not be complicated GeneSys image capture software from Syngene makes imaging gels and western blots simple using system protocols – just place your sample in the darkroom, position your sample and hit capture for high quality images.

4

Environmentally friendly

Implementing a gel imaging system eliminates the chemical waste associated with film processing. Select a system that uses LEDs as they are environmentally friendly.

Need more help? Ask one of our distributors who will be able to provide specific answers and recommendations, including pricing.

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