Friday 1st October 2010

National Heart & Lung Institute

G:BOX Chemi HR16 is being used at the National Heart & Lung Institute to assist in identifying proteins associated with cardiomyopathy.

How we use the G:BOX Chemi HR16 system

Researchers at the NHLI are using the G:BOX Chemi HR16 system fitted with a 9-position filter wheel to visualise 1D gels of proteins stained with Pro-Q® Diamond, SYPRO® Ruby and Pro-Q® Emerald (Invitrogen). These images are then being analysed with GeneTools, Syngene’s market leading software to quantify the amounts of phosphorylated and glycosylated proteins present in diseased human and mouse heart tissues. The results of this research could ultimately help to determine which proteins are associated with different cardiac diseases.

Excellent sensitivity

Dr Clare Gallon, a Research Associate in Cardiac Medicine at the NHLI said: ”We routinely use the G:BOX Chemi HR16 many times a day for imaging a variety of fluorescently stained proteins from cellular heart extracts. The system provides excellent sensitivity allowing us to see even very small amounts of expressed proteins, and using the GeneTools software, image analysis is rapid, making it easy for us to obtain accurate results immediately. We are so pleased with the performance of the G:BOX Chemi HR16 that we have had a Qdot filter fitted to extend the system’s future application range to imaging different coloured Qdot labelled proteins on single Western blots”.



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