The G:BOX Chemi IR6 is a fully configured gel documentation (gel doc) system for infrared (IR) applications. This high specification model easily out-performs laser based systems in terms of capability, and more importantly, at a fraction of the price.
G:BOX Chemi IR6 has a 6m pixel cooled CCD camera for superb image quality and a lighting system for the infrared imaging of IR dyes such as DyLight 680 and DyLight 800. Other brands of IR dyes can also be imaged with G:BOX Chemi IR6, with similar results. The system has comparable sensitivity and has a limit of detection (LOD) the same, if not better, than the most popular laser based systems and high resolution to compete at the top end of these laser systems.
Other advantages of the G:BOX Chemi IR6 is that unlike laser based systems it can work across a very wide range of applications such as, DNA gel staining, blue light applications, Northern/Southern/Western blots, multiplexed Western blots, EMSA/gel shift assay, protein gel staining and many more without the need to change your standard protocols within the laboratory.
The G:BOX Chemi IR6 is ideal for chemiluminescence work and with the upgrade to the edge lighting unit, the system can also be used for 2D electrophoresis gels. This is truly a versatile package which would make an ideal workstation for the busy laboratory doing a wide range of applications.
The system comes complete with unlimited copies of GeneTools analysis software.
All trademarks acknowledged
Perfect for IR applications
A versatile system
A sensible price
|CCD camera (m pixels)||6.3|
|Bit depth (grey scales)||16 bit/65,536|
|Dynamic range||4.8 OD|
Ultra peltier cooling with forced air for extra long exposures
|Lens (motor driven with feedback)|
|Built-in white light||Yes|
|Filter wheel motor driven||Yes with UV orange filter, FILT 705(M), FILT LY800(M)|
|Size 20x20cm or 25x30cm
|UltraBright-LED blue 20x16cm||Optional|
|UV/visible Novaglo converter||Optional|
|LED Red(M), LED IR740(M)||Yes|
|LED Green, Blue||Optional|
|Dimensions (cm)||57 x 100 x 52|
The biggest difference between a Syngene system and any other is in the way the system is controlled. At the heart of all Syngene systems is GeneSys which is an application driven control software. With G:BOX you can use GeneSys in either Automatic or Manual mode.
It is assumed that the user will know exactly what their application is and how they have prepared their gel or blot. In Auto Mode they simply enter this information into GeneSys (or recall it from a saved configuration) and the system takes over the rest. Behind GeneSys is an extensive database which contains data relating to a very wide range of applications, eg, fluorescence, chemiluminescene and chemifluorescence. Once the G:BOX has been told what sample to expect then GeneSys decides what hardware configuration is best and sets the system ready for image capture. Things like camera control, exposure time, sensitivity setting, lighting requirements, lens control, filter selection – all of this is taken care of by GeneSys. The user just has to click the ‘capture’ button and wait for the perfect image to appear.
From the opening screen the user can select the mode of operation – Auto or Manual. It is also possible to access the image browser and enter the edit screen to work on previously stored images. Previously stored configurations are shown on the left as small ‘notebooks’.
In Auto Mode the user simply selects the ‘sample format’, the ‘sample type’ and the ‘matrix type’. The software has been designed for use with a touch screen.
Once the application type has been selected GeneSys then offers the user a range of dyes from its database. The user then simply selects the dye. It is also possible to undertake multiplex applications in which case the user would select each dye from the available list. The database behind GeneSys contains over 210 different dyes and stains and is updated on a regular basis as more options come onto the market.
Once the dye has been selected then the system will automatically configure itself to capture the best possible image of the sample. The lighting, filter and camera exposure settings are all automatically adjusted for the sample type. The captured image is displayed in the main screen with small thumbnail images of the current and previous images (either opened or captured) shown in an image ‘pool’. Note the icons at the foot of the screen which enable previous pages to be selected.
The fully automatic functions of GeneSys allow even the most difficult or complicated gels and blots to be imaged. For example with Chemiluminescence the system uses its intelligence to know the output profile of most of the major substrates. It can then decide the exposure setting best suited to a particular substrate. Then during the image capture process it continually monitors the output from the blot until the best image is produced.
Another example of the power of GeneSys is when working with multiplex blots. Once the application information is selected by the user GeneSys will make separate exposures using the correct filter, lighting and camera setting for each dye. The final multiplex image will be displayed.
On the image edit page the user can enhance the image, add annotation, save or print.
Not everyone wants their system to take automatic control. Therefore, there is the ability for the user to take complete control of every function of the system by using the manual mode GeneSys has been designed using the most advanced software tools available. Primarily it has been designed for use with a ‘touch’ panel PC and to take advantage of the new functions afforded by Windows 7. Selection of all options using the simple interface is easy and intuitive.
Blue light transilluminators are often used as an alternative to a UV transilluminator when users wish to use ‘safe dyes’ instead of Ethidium Bromide. A blue light transilluminator is a ‘safe’ light source, in that the user is not exposed to harmful UV radiation and samples are free from photonicking. The UltraBright-LED transilluminator can be used instead of the UV transilluminator in all Syngene G:BOX systems.
UltraBright-LED transilluminator can be used with gels up to 20 x 16cm for use in the G:BOX series. This model uses side mounted 470nm LED arrays for illumination.
Please refer to the Syngene database for the comprehensive list of dyes which can be used with this transilluminator.
The Epi UV module can be fitted to any G:BOX IR6 darkroom (those produced after July 2010). The module fits above the standard LED white light module which is standard on all G:BOX IR6 systems. The Epi UV module can have either a 254nm, 365nm or 302nm tube. Either one or two modules can be fitted to each darkroom.
Please refer to the Syngene database for the comprehensive list of dyes which can be used with the Epi light module.
The filter wheel takes the 58mm range of filters.
Please refer to the Syngene database for a more comprehensive list of dyes which can be used with each filter.
For a more versatile G:BOX IR6 system, LED light modules for red, green and blue light can be fitted to an optional lighting gantry.
With this new lighting system the user can select which wavelength of light they require for any specific application. The lighting modules are single wavelength and contain LEDs and a dedicated filter. Each module is "plug and play". Once plugged into the gantry the module is electronically registered by the G:BOX IR6 which recognises the wavelength and type of filter being used.
Up to 4 modules can be plugged into each side of the darkroom. It is possible to fit identical modules to increase intensity or to use a number of modules with different wavelengths. Modules are normally fitted in matched pairs, one either side.
There are 8 different LED modules to choose from:
Module Wavelength Filter type Applications
Blue 465 Cut off General
Blue (m) 465 Band pass Close range multiplex apps
Green 520 Cut off General
Green (m) 520 Band pass Close range multiplex apps
Red 635 Cut off General
Red (m) 635 Band pass Close range multiplex apps
IR 740 Cut off General
IR (m) 740 Band pass Close range multiplex apps
Please refer to the Syngene database for the comprehensive list of dyes which can be used with the LED light modules.
Any printer with appropriate Windows drivers can be attached to the G:BOX IR6. We recommend an A6 thermal printer such as Sony or Mitsubishi. These USB printers will "plug and play" with the G:BOX IR6 and require minimal set up. They will print hard copies of images in under 5 seconds on long life glossy thermal paper to give photographic quality images.
More and more laboratories require IQ/OQ/PQ verification these days. Syngene’s IQ/OQ/PQ procedures take the user through each step in the verification of a Syngene system in meeting the demands of the laboratory, regulatory compliances and standard operational procedures (SOP). These simple procedures allow the user to verify that a system meets defined installation, quality and performance specifications.
Installation Qualification (IQ)
This verifies that when a system is unpacked it conforms with all purchasing, shipping and unpacking documents. Once this procedure has been followed the system is then ready for the operational check process.
Operational Qualification (OQ)
This procedure establishes if the system meets all the operation and functionality specifications. An operational test is also included at this stage.
Performance Qualification (PQ)
A performance check will establish if the system meets the specification for the nature of the purpose it was intended for. This is verified by using a simple target to establish if the system can meet performance criteria.
Each set of IQ/OQ/PQ documents contains a complete set of procedures and checklists for following the verification steps. Included with each ‘pack’ is a simple calibration device (target image) so that the performance of the system can be checked against the PQ requirements. Each set of documents is unique to a particular system and has to be ordered with the system itself. The document pack has full instructions for use and implementation.
Who does this?
Customer - typically a customer can perform these tests by themselves. Their own internal QC department will be familiar with these processes.
Syngene or its distributors
Trained Syngene personnel or trained distributors can also perform the IQ/OQ/PQ procedures, normally at a nominal charge.
Copyright © 2009-2011 Syngene, A Division of Synoptics Ltd