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Polysaccharide Analysis Using Carbohydrate Gel Electrophoresis
A method to characterize plant cell wall polysaccharides is presented. The complexity of the polymer structures and the large number of different charged and uncharged monosaccharides that make up plant polysaccharides have previously made analysis technically demanding and laborious. Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of reducing ends of sugars and oligosaccharides with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels.
GeneSnap and GeneTools
Please see article entitled "A New Constitutively Active Brain PAK3 Isoform Displays Modified Specificities toward Rac and CdC42 GTPases" published in The Journal of Biological Chemistry, issue of February 7 2003. This article mentions both GeneSnap and GeneTools.
Riboprobes imaging process brings benefits to spinal injuries research
With around 90,000 people worldwide incurring a spinal cord injury each year, it is estimated that by 2005 the total world population living with such an injury will rise to over 2.5 million. Spinal cord injury is a major problem because damage to the spinal cord is irreversible. Injured nerves fibres do not normally re-grow, so communication with other nerve cells is lost. One reason why nerves cannot regenerate after a spinal cord injury is because a glial scar is formed around the injury producing a physical barrier. In addition, after injury some inhibitory molecules, such as guidance or matrix proteins are up-regulated, these molecules impair any possibility of axon repair and therefore of recovery. (Thanks to ISC for providing Syngene with the pdf of this article). This article cites the use of GeneGenius.
Neutral Fielding: a background correction method for large gel analysis
The accuracy of quantitative measurements based on the image analysis of gel patterns is directly dependent on the uniformity of the light illuminating the gel. Particularly with large gels, there can be a significant variation of such illumination across the gel. A novel software correction system, known as neutral fielding, can compensate for such background variability.
Regulation of Cre recombinase by ligand-induced complementation of inactive fragments
There is a reference to both ChemiGenius2 and GeneTools in the Materials and Methods (Northern and Southern blotting section) page 2.
Estrogen Regulation of the Cytochrome P450 3A Subfamily in Humans
There is a reference to both ChemiGenius2 and GeneTools in the following article.
Imaging Fluorescent Proteomics Dyes
The visualization of proteins on polyacrylamide gels is usually performed by utilizing Coomassie Blue (J.T. Baker, Deventer, The Netherlands)or silver staining. New fluorescent dyes for staining proteins offer such benefits as increased sensitivity and reproducibility over a broad dynamic range(1), as well as mass spectrometry compatibility(2). In addition, they provide simple staining protocols, are easier to dispose of, and are more environmentally friendly than silver staining.
Iron release from macrophages after erythrophagocytosis is up-regulated by ferroportin 1 overexpression and down-regulated by hepcidin
There is a reference to both GeneFlash and GeneTools in the following article.
Lactacystin-induced apoptosis of cultured mouse cortical neurons is associated with accumulation of PTEN in the detergent-resistant membrance fraction
ChemiGenius2 was used to obtain some of the results in the following article.
Detecting Fluorescent Dyes on 1-D Polyacrylamide Gels Gels using CCD-based Technology as Compared to Laser Scanning
Laser scanners are commonly used to detect some of the newer fluorescent protein stains such as Sypro Ruby (Molecular Probes, Inc., Leiden, The Netherlands), because it provides detection in the nanogram range. However, it does have major drawbacks including being an expensive technology, with limited uses in terms of detecting a range of visible protein stains. Charge-coupled device (CCD) technology offers the advantage of being more flexible in its applications but is often reported as being less sensitive than laser scanning for measuring proteins labeled with fluorescent dyes. This article appeared on www.genprogmag.com
Gold Aids Visualisation
Gold-stained immunoblots allow rapid and safe analysis of 200kD disease-associated proteins.
An investigation of pectin methylesterification patterns by two independent methods: capillary electrophoresis and polysaccharide analysis using carbohydrate gel electrophoresis
There is a reference to GeneTools in the following article.
Automated Imaging of Large Gold-Labelled Western Blots. How this is Helping Research in Type 2 Diabetes
According to the WHO, there are around 150 million cases of type 2 diabetes world-wide and its prevalence is expected to double over the next 15 years. Therefore, finding new therapies to protect against this disease is becoming of increasing importance.
Journal of Cell Biology article
There is reference to Syngene in the following article.
Comparison of nuclear matrix proteins between gastric cancer and normal gastric tissue
There is reference to Syngene in the following article.
Heparan sulfate regulates amyloid precursor protein
There is reference to Syngene in the following article.
Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression
There is reference to Syngene in the following article.
Resolution of the structural isomers of partially methylesterified oligogalcturonides by polysaccharide analysis using carbohydrate gel electrophoresis
There is reference to GeneTools in the following article.
Sustained antiproliferative mechanisms by RB24, a targeted precursor of multiple inhibitors of epidermal growth factor receptor and a DNA alkylating agent in the A431 epidermal carcinoma of the vulva cell line - British Journal of Cancer
There is a reference to Syngene and GeneTools in the following article.
Genomic Characterization of an Endemic Mycobacterium tuberculosis Strain: Evolutionary and Epidemiologic Implications
There is a reference to Syngene and GeneTools in the following article.
Expression of fnr Is Constrained by an Upstream IS5 Insertion in Certain Escherichia coli K-12 Strains
There is a reference to Syngene and GeneTools in the following article.
Herpes simplex virus interferes with amyloid precursor protein processing
There is a reference to Syngene and GeneTools in the following article.
Design of a Chimeric 3-Methyl-1,2,3-triazene with Mixed Receptor Tyrosine Kinase and DNA Damaging Properties: A Novel Tumor Targeting Strategy
There is a reference to Syngene and GeneTools in the following article.
Pulmonary antioxidants exert differential protective effects against urban and industrial particulate matter
There is a reference to Syngene and GeneTools in the following article.
Erectile dysfunction: an early marker for hypertension?
There is a reference to Syngene and GeneTools in the following article.
Synaptic thermoprotection in a desert-dwelling drosophila species
There is a reference to Syngene and GeneTools in the following article.
Insulin receptor expression during development and a reproductive cycle in the ovary of the mosquito
There is a reference to GeneGnome and GeneTools in the following article.
Posttranslational modifications of p27 kip1 determine its binding specificity to different cyclins and cyclin-dependent kinases in vivo
There is a reference to GeneTools in the following article.
Hepcidin Decreases Ferroportin 1 Expression in a Mouse Macrophage Cell Line
There is a reference to GeneFlash and GeneTools in the following article.
Intestinal ischemic preconditioning in rats and NF-B activation
There is a link to GeneTools in the following article.
Estrogen Regulation of the Cytochrome P450 3A Subfamily in Humans
There is a link to GeneTools in the following article.
Cloning and characterization of chicken stromal cell derived factor-1
There is a link to GeneTools in the following article.
Inhibition of cell signaling by the combi-nitrosourea FD137 in the androgen independent DU145 prostate cancer cell line
There is a link to GeneTools in the following article.
Characterization of the role of the AMP-activated protein kinase in the stimulation of glucose transport in skeletal muscle cells
There is a link to GeneTools in the following article.
Linking microbial genetic- and functional diversity in compost: DGGE-profiling separated different types of compost regarding chitinase activity
There is a link to GeneTools and GeneSnap in the following article.
Plant derived products as a source of cellular growth inhibitory phytochemicals on PC-3M, DU-145 and LNCaP prostate cancer cell lines
There is a link to GeneTools in the following article.
Cell cycle-dependent nuclear retention of p53 by E2F1 requires phosphorylation of p53 at Ser315
There is a link to GeneTools in the following article.
The Antiarrhythmic Peptide Rotigaptide (ZP123) increases Connexin 43 Protein Expression in Neonatal Rat Ventricular Cardiomyocytes
There is a link to GeneTools in the following article.
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