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Visualising the future of research
Image processing used to be the bottleneck in scientific research, but times are changing, as David Robson discovers.
The following article appeared in Imaging and Machine Vision Europe: February/March 2008 and mentions Dyversity.
Ibogaine - indole alkaloid of African plant Tabernanthe iboga shows antioxidative properties
This poster was presented in the Havana - Redox 2007 Conference in Cuba. Dymension was used in the analysis of the gel image.
Nanoparticle-based detection and quantification of DNA with single nucleotide polymorphism (SNP) discrimination selectivity
Sequence-specific DNA detection is important in various biomedical applications such as gene expression profiling, disease diagnosis and treatment, drug discovery and forensic analysis. Here we report a gold nanoparticle-based method that allows DNA detection and quantification and is capable of single nucleotide polymorphism (SNP) discrimination. The precise quantification of single-stranded DNA is due to the formation of defined nanoparticle-DNA conjugate groupings in the presence of target/linker DNA. Conjugate groupings were characterized and quantified by gel electrophoresis. A linear correlation between the amount of target DNA and conjugate groupings was found. For SNP detection, single base mismatch discrimination was achieved for both the end- and center-base mismatch. The method described here may be useful for the development of a simple and quantitative DNA detection assay.
This article cites the use of GeneGenius.
Ibogaine affects brain energy metabolism
This paper appeared in European Journal of Pharmacology 552 (200) 11-14 and cites the use of Dymension in the analysis of the gel image.
Antioxidative action of royal jelly in the yeast cell
This paper appeared in Experimental Gerontology 42 (2007) 594-600 and cites the use of Dymension in the analysis of the gel image.
CCD-based system offers rapid method for detecting glycosylated and non-glycosylated proteins
Glycosylation is the most common form of post-translational modification of human and other eukaryotic proteins. Glycoproteins have vital roles in many physiological and pathological reactions and are also responsible for molecular recognition, cell signalling and protein stability, as well as protein folding and structure. Therefore, identifying glycoproteins is often of critical importance in life science research.
This article cites the use of Dyversity 4.
Imaging fluorescently stained DNA with CCD technology. How to increase sensitivity and reduce integration times
In this article we describe how using CCD technology with a blue converter screen and the correct lighting and filter conditions enables scientists to rapidly image SYBR Green and SYBR Gold stains with better levels of sensitivity than DNA stained with ethidium bromide, thus offering a safe yet accurate imaging method.
This article appeared in Biotechniques (March 2007 issue) and cites the use of G:BOX Chemi HR16.
Using a CCD-based imager to scan PACE gels provides a quick and sensitive method of analysis
Analysis of polysaccharides can be problematic because of the need for complex methods and/or expensive equipment such as chromatography and mass spectrometry, NMR, capillary electrophoresis and Fourier transform infrared spectroscopy. These techniques are ideal for a particular polysaccharide or application, but they have limitations, such as the need for pure compounds or relatively large sample quantity.
Recently, a method known as PACE (polysaccharide analysis using carbohydrate gel electrophoresis) has been described. This can be used in a high throughput manner and is applicable to small quantities of material. This article describes how CCD-based technology can be used to detect the fluorescent signal produced on a PACE gel and the sensitivity this type of system can achieve.
This article appeared in International Labmate (February 2007 issue) and cites the use of G:BOX Chemi HR16.
CCD technology compared with laser-based scanning for analysing fluorescently labelled proteins
The use of fluorescent labelling has driven the need for equipment that can rapidly detect and analyse fluorescent protein bands and spots. Currently Charged Coupling Device (CCD) image analysers and laser scanners have emerged as capable of meeting these technically challenging demands and this article details how these two technologies compare in terms of sensitivity and speed.
This article appeared in BioTech International (November 2006 issue) and cites the use of Dyversity.
Proteomics: challenges and emerging technologies, EuroSciCon
This is a meeting report which was published in Expert Review of Proteomics, December 2006 , Vol. 3, No. 6, pages 573-577 and is the copyright of Future Drugs Ltd.
A New Method for Rapidly Visualising Multiple Different Proteins on Western Blots
Western blot analysis is a widely used technique for detecting expression of specific proteins. However, many current methods of labelling Western blots are unsuitable for detecting different proteins on the same blot (multiplex detection) because they only produce one colour or chemiluminescent emission. Using a new protocol known as FluoWestTM, we describe here how the use of Qdot-labelled secondary antibodies coupled with a G:BOX Chemi CCD-based image analyser can be used to detect and produce an image of three different types of protein on one blot.
This article appeared in Drug Plus International (June/July 2006 issue).
Accurate Identification of Plants That Cause Subsidence Damage
A Practical Application of Genetic Data Analysis using GeneDirectory
Accurate analysis of tree or shrub roots is often crucial in the outcome of subsidence claims where plants are implicated with damage to a building. In fact, insurers often make decisions on claims based on this information to obtain compensation from the owner of the plant in question.
Automated Imaging of Western Blots and Clonogenicity Assays
AstraZeneca, one of the world's top five pharmaceutical companies, currently spends over £5 million each working day on global research and development of new therapeutics for a range of diseases. One of the Company's major research targets is to deliver more than 15 candidate drugs annually by 2003. To reach this kind of target, AstraZeneca needs to constantly find ways of improving the speed and accuracy of its pre-clinical research processes. An example of where the Company is currently doing this is in one of its Cancer Research Groups at Alderley Park in Cheshire.
This article cites the use of ChemiGenius.
Chemiluminescent Detection of Nucleic Acids
Chemiluminescence has been used for some years as an alternative to radioactive labelling for the detection of proteins and nucleic acids. However, although chemiluminescent reagents have undergone many improvements in terms of sensitivity and speed since their first introduction, the disadvantages of film-based visualisation methods have restricted the wide-scale use of chemiluminescence.
This article cites the use of GeneGnome.
Investigations of EGF Receptors in Ovarian Cancer Cell Lines Using Chemiluminesence Detection for Gel Electrophoresis
The use of chemiluminescence indicators in gel electrophoresis applications has become increasingly popular in recent years . Chemiluminescence indicators can provide a useful alternative to radioactive indicators and do not suffer from the health and safety concerns associated with the use of radioactive material. Chemiluminescence output, however, is inherently weak in intensity and is most frequently detected by exposure to film over an extended period of time. This application note introduces ChemiGenius, a specially developed gel documentation and analysis system which combines the use of an automatic analysis system and a high sensitivity cooled CCD camera for direct imaging of chemiluminescence emissions.
GeneGnome and GeneGenius
Please see Application Note entitled "Separation and purification of hemoglobin using modified polyethylene glycose 6000 mixed with a liquid-solid extraction system of polyethylene sorbitan monooleate and aqueous K2HPO4-KH2PO4" on page 24 of American Biotechnology Laboratory December 2002. This article mentions both GeneGenius and GeneGnome.
Automated Image Analysis - How it can Improve Accuracy and Increase Productivity in Drug Discovery
Pharmaceutical and biotechnology companies currently spend millions of pounds each working day on global research and development of new therapeutics for a range of diseases. Many of these firms have highly demanding research targets and aim to deliver as many as 15 candidate drugs annually. Nowhere is this felt more keenly than by those involved in genomics and proteomics research for drug discovery. Since these are relatively new fields, they still have to prove they can help to generate good clinical candidates. Therefore, these groups are constantly trying to find ways of improving the speed and precision of their pre-clinical research processes in order to rapidly deliver promising results. This is where automation can help to improve accuracy and reduce substantial bottlenecks in areas such as quantification of nucleic acids and proteins, as well as counting recombinant clones.
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