GeneTools functions

GeneTools software aids 21 CFR, Part 11 compliance

1D analysis

Auto track finding - NO limit - using a single click
Auto band finding
Auto edge finding
Manual override of all automated functions if required
Auto baseline correction - 5 different methods including manual selection
Track finding in ANY direction - down, up, left to right, right to left
Multi-tier analysis for high throughput screening
Peak detection parameters can be altered to suit range of applications
Unlimited track width setting
Auto track, band and edge numbering and identification with user defined colours
Track labelling
Track histogram alongside image for easy identification
Manual band addition and deletion with recording for GLP purposes
Track profile analysis - unlimited track overlays
Molecular weight calibration curve display
Quantity calibration curve display
User configurable results of: position, distance travelled, peak height, area, raw volume, % raw volume, Rf, MW, Quantity, calibration standard, peak width

Molecular weight

Library of markers for automatic or manual assignment
User configurable library
Unlimited number of markers can be used per gel
Auto interpolation of multiple markers for use with smiling or distorted gels
Curve type, calibration method selection (log linear fit, log piecewise linear)
Calibration curves are displayed

Quantity

Manual input of calibrated standards and quantities
Various linear or quadratic calibrations can be selected

Spot blot

Optional spot or slot blot
Works in grids or non grid formats
Random spot/slots automatically located
Auto detection of absorption or fluorescence
Ability to set all spot/slots to one size or auto detection with variable size
Auto compensation for grid variances
Thresholding of spots
Manual control of spot sizes, positions and grid position
Background corrections – manual or auto
No limit to the number of manual background subtractions
Auto selection of nearest background for subtraction
Incidence parameters for positive and negative reactions with both 'greater than' and 'less than' values or a range of values can be set
Incidence value can be set using raw volume, percentage of total, pixel area of spot/slot, calibrated quantity value
Volume calibrations
Display of calibration curves

Colony counting

Superb NEW colony counting function
Auto counting of colonies with manual editing capability
Ability to count light and dark colonies on the same plate as well as two colour colonies at any one time
Ability to vary the position and size of area of interest
Unique auto-separation of colonies
Proprietary algorithm gives near 100% accuracy - therefore no need for an adding or subtraction, manual editing function
Variable sensitivity for detection
Ability to set min and max colony areas
Class split for two colour colonies giving count and % area of plate covered
Ability to show shape of detected colony
Manual X and Y positions
Brightness, contrast and Gamma control
Define areas of exclusion – also adjustable
Results table

Arrays and plates

Total auto detection of spot patterns without the need for manual skewing
Detection of spot patterns at any angle without need for adjustment
Auto background subtraction, no need for a blank background subtraction
Immediate export into Excel of all data for comparison information

Gel scoring

Auto positive and negative and presence and absence of bands
Results shown as table

Band matching

GeneTools has band matching facilities. This gives the user the ability to align bands, determine track relationships and compare track data. This is all achieved automatically but with the added advantage of manual override.
Band matching by position, defined Rf or calibrated molecular weight
Tolerance variation (%)
Auto match function
Dendrogram, cluster analysis
Choice of analysis and display algorithms
Matching comparisons (presence and absence)
Matching matrix
Matching coefficients

Manual band quantification

This section of GeneTools allows the user to manually quantify bands, spots or other objects on a sample. The drawing tool may be rectangular or free-hand, giving total flexibility. Once all areas of interest are specified, a background correction may be applied automatically or manually selected from the surrounding sample. The user is able to quantify and perform threshold analysis using positive and or negative controls. Standard curve data may also be entered. All data is instantly exportable to Excel ® .