Which excitation wavelength and which emission filter should I use? 1. Select your system 2. Select your dye 3. Select your sample format Choose Dyversity G:Box Chemi range using cooled cameras G:Box range using non cooled cameras GeneGnome InGenius Choose Acridine Orange - DNA bound Acridine Orange - RNA bound Alexa Fluor 350 Alexa Fluor 405 Alexa Fluor 430 Alexa Fluor 488 Alexa Fluor 500 Alexa Fluor 514 Alexa Fluor 532 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 568 Alexa Fluor 594 Alexa Fluor 610 Alexa Fluor 610 R-phycoerythrin streptavidin Alexa Fluor 633 Alexa Fluor 635 Alexa Fluor 647 Alexa Fluor 647-R-phycoerythrin streptavidin Alexa Fluor 660 Alexa Fluor 680 Alexa Fluor 700 Alexa Fluor 750 AMAC AMC/Coumarin AMCA/AMCA-HPDP Amido Black ANTS APC/Allophycocyanin AttoPhos BlueView BODIPY 630/650-X BODIPY 650/665 BODIPY FL BODIPY R6G BODIPY TMR-X BODIPY TR-X Chemifluorescence Chemiluminescence Chromogen/BCIP/NBT Colorimetric stains Coomassie Blue and variants Coommassie Fluor Orange Copper stain Crystal violet Cy2 Cy3 Cy3.5 Cy5 Cy5.5 Cy7 DAPI DDAO Phosphate Deep Purple DyeChrome for Amplex Gold reaction product DyeChrome for BODIPY FL-X reaction product DyeChrome for DDAO reaction product DyeChrome for MDPF reaction product DyeChrrome for ELF 39 reaction product DyLight 405 DyLight 488 DyLight 549 DyLight 633 DyLight 649 DyLight 680 eBFP/Enhanced blue fluorescent protein eCFP/Enhanced cyan fluorescent protein ECL chemiluminescence substrates ECL Plus (fluorescent excitation) eGFP/Enhanced green fluorescent protein EnVISION Ethidium bromide Ethidium homodimer EviTag Birch Yellow EviTag Catskill Green EviTag Fort Orange EviTag Macoun Red EviTag Maple Red-Orange eYFP/Enhanced yellow fluorescent protein EZ-ECL chemiluminescence substrates EZ-VISION F1AsH FAM Fast Blast FITC/fluorescein isothiocyanate Flamingo GelGreen GelRed GelStar Glyco-Stain Hoechst Blue/33258 InVision His-tag In-gel Invitrogen Western Breeze chemiluminescence s IRDye 680 JOE Krypton Protein Stain LavaPurple Lucy-506 Lucy-565 Lucy-569 Lumitein MegaFluor MemCode Methylene Blue Millipore Immobilon Western chemiluminescence Monobromobimane Nile blue A Nile red Oregon Green 488 Oregon Green 514 Pacific Blue Pacific Orange Phos-tag 300/460 Phos-tag 540 Pierce chemiluminescence substrates Ponceau S Pro-Q Diamond Pro-Q Emerald 300 Pro-Q Emerald 488 Pro-Q Sapphire 365 Pro-Q Sapphire 488 ProGreen ProQ DDAO ProRed Qdot 525 Qdot 565 Qdot 605 Qdot 625 Qdot 655 Qdot 705 Qdot 800 QUIKView Radiant Red ReAsH Reverse staining RFP/DSRed/Red fluorescent protein Rhodamine 123 Rhodamine 6G Rhodamine B Rhodamine Green Rhodamine Phalloidin Rhodamine Red Rhodol Green ROX SafeView - dsDNA bound SafeView - ssDNA and RNA bound Silver stain and variants SuperSignal chemiluminescence substrates SYBR Gold SYBR Green I SYBR Green II SYBR Safe SYPRO Orange SYPRO Red SYPRO Rose Plus SYPRO Ruby SYPRO Tangerine SYTO 11 SYTO 12 SYTO 13 SYTO 14 SYTO 15 SYTO 16 SYTO 17 SYTO 18 SYTO 20 SYTO 21 SYTO 22 SYTO 23 SYTO 24 SYTO 25 SYTO 45 SYTO 59 SYTO 60 SYTO 61 SYTO 62 SYTO 63 SYTO 64 SYTO 9 SYTO BC TAMRA Texas Red Vistra Green wtGFP/Wild type green fluorescent protein X-ray film densitometry Xeno Fluor 750 Zinc stain Choose Opaque Membrane/Blot (PVDF, nitrocellulose) Translucent Gel (agarose) Transparent Gel (acrylamide) Multiplex applications have not been considered, so if you need to multiplex a number of dyes then please contact Syngene for further information. If you are unsure of the emission filter chosen for your chosen dye and require a custom filter as you would like to multiplex, then please consult Syngene for the design of custom filters. If you select a visible dye, depending on the excitation light you use, you may need to select the UV filter or ND filter depending on whether you would like to improve the contrast or reduce saturation. Only the 6 most popular chemiluminescent kits are listed; if your kit is not listed then choose “chemiluminescence”. There are many silver and coomassie blue variants, and these have been grouped under “Coomassie blue and variants” and “Silver and variants”. There are many colorimetric reagents so these have been grouped under “Colorimetric dyes”. If your dye of interest is not listed then contact Syngene.

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