Friday 5th November 2010
Scientists in the Department of Anesthesiology at DHMC are using a G:BOX Chemi HR16 to image Western blots of HRP labelled proteins that are involved in inflammatory processes, (p-ERK, total-ERK, MKP1, NOS2, IL-1 and TNF) isolated from neonatal cortical microglial cells treated with new drugs, post peripheral nerve injury. By identifying a protein’s presence, DHMC researchers hope to determine which drugs most effectively treat the pain caused by peripheral nerve injury.
G:BOX Chemi HR16 improved the sensitivity of imaging Western blots
Dr Edgar Alfonso Romero-Sandoval, Instructor of Anesthesiology at the DHMC explained: “Pain following peripheral nerve injury often responds poorly to available therapies, which is why we are looking at new drugs to treat it. To do this we use expensive rat primary cell cultures that yield little protein and were using a laser scanner to image our Westerns but could not detect the microgram amounts of proteins we had, as there was so much background.”
Why we chose Syngene
Dr Romero-Sandoval commented: “We switched to a G:BOX Chemi HR16 because it allows us to adjust exposure conditions, (something we couldn’t do with our laser scanner) to detect these tiny protein amounts. Using a G:BOX Chemi HR16 has improved the sensitivity of imaging our Western blots so much, we have been able to reduce the detecting antibody concentration by 2-4 fold and significantly lowered reagent costs. Additionally, with a G:BOX we can strip our membranes several times and are still able to detect different proteins using the same blot derived from the same experiment and cell culture.”
Copyright © 2009-2016 Syngene, A Division of Synoptics Ltd