Dyversity is designed for the rapid generation of 2D protein gel images and features a high resolution, 16-bit CCD camera inside a computer-controlled darkroom. This camera offers unrivalled separation of the highest density of protein spot images and can capture faint spots guaranteeing imaging accuracy which is comparable to many expensive laser based scanning systems.
The versatile Dyversity system has a motor driven sample stage, motor driven lens and can be fitted with a range of filters, UV and visible lighting options to allow imaging of all commercial 2D protein stains including Coomassie Blue, silver stain, SYPRO® Ruby, Pro-Q® Diamond, Deep Purple™. With an optional lighting module it is also possible to image gels stained with Cy2, Cy3 and Cy5. The high-quality 2D gel images produced by Dyversity can be easily downloaded for rapid analysis into Dymension, Syngene's powerful 2D gel image analysis software.
|Pixel array||3072 x 2048|
|Bit depth (grey scales)
65,536 grey levels
|F1.4 motor driven with feedback
|Motor driven stage||Yes|
|Built-in white light||Yes - LEDs|
|Filter wheel||7 position with included UV orange filter|
|UV transilluminator 20x20cm or 25x30cm||Option|
|UltraBright-LED blue 20x16cm||Option|
|Edge lighting module RGB||Option|
|Epi UV 254, 302 or 365nm||Option|
|Epi red, green, blue, IR||Option|
|UV/visible light Novaglo converter||Option|
|Dimensions (wxhxd)||57 x 85 x 43|
Blue light transilluminator
Used when alternative dyes to EtBr are required. A blue light transilluminator is a "safe" light source in that the user is not exposed to harmful UV radiation and samples are free from photonicking. This transilluminator replaces the UV transilluminator in all products.
Please refer to the Syngene database for the comprehensive list of dyes which can be used with this transilluminator.
The Epi UV module can be fitted to the Dyversity darkroom (those produced after July 2010). The module fits above the standard LED white light module which is standard on all Dyversity systems. The Epi UV module can either have a 254nm, 365nm or 302nm tube. Either one or two modules can be fitted to each darkroom.
Please refer to the Syngene database for the comprehensive list of dyes which can be used with the Epi light module.
The filter wheel takes the 58mm range of filters.
Please refer to the Syngene database for a more comprehensive list of dyes which can be used with each filter.
To increase the number of applications for which the Dyversity can be used it is possible to fit LED light modules for red, green, blue and IR light.
With this new lighting system the user can now select which wavelength of light they require for any specific application. The lighting modules are single wavelength and contain LEDs and a dedicated filter. Each module is "plug and play". Once plugged into the gantry the module is electronically registered by the Dyversity which recognises the wavelength and type of filter being used.
Up to 4 modules can be plugged into each side of the darkroom. It is possible to fit identifical modules to increase intensity or to use a number of modules with different wavelengths. Modules are normally fitted in matched pairs, one either side.
There are 8 different LED modules to choose from:-
Module type Wavelength (nm) Filter type Applications
Blue 465 Cut off General
Blue (m) 465 Band pass Close range multiplex apps
Green 520 Cut off General
Green (m) 520 Band pass Close range multiplex apps
Red 635 Cut off General
Red (m) 635 Band pass Close range multiplex apps
IR 755 Cut off General
IR 780 Band pass Close range multiplex apps
Please refer to the Syngene database for the comprehensive list of dyes which can be used with the LED lighting modules.
Edge lighting module
A range of wavelengths and filter sizes are available.
||White light converter
White light converters for converting UV or blue light to white for viewing visible gel stains.
Any printer with appropriate Windows drivers can be attached to the Dyversity. We recommend a thermal A6 printer such as Sony or Mitsubishi. These USB printers will "plug and play" with the Dyversity and require minimal set up. They will print hard copies of images in under 5 seconds on long life glossy thermal paper to give photographic quality images.
“Since 2007, we have been using the Dyversity 2D image analysis system to analyze proteins associated with brain tumors on chemiluminescent Westerns and have detected and quantified bands which we haven’t been able to see using other image analyzers. We also use Dyversity with Dymension 2D analysis software to quantify protein spots on 2D gels because the two together make this complicated analysis straightforward.”
“The technical support we have had from Syngene is excellent. They have taught us how to use the software efficiently and have always been prompt to respond to all our queries with helpful suggestions. I would go so far as to say we have never had such great support from any other company and I would recommend a Dyversity and Dymension to anyone thinking of doing 2D gel work.”
“Our Dyversity system is used by a number of different research groups to image chemiluminescent and fluorescent Westerns, as well as DNA and protein gels of a variety of cancers. The service we have had from Syngene is excellent, as they trained us to get the best out of the Dyversity’s software and even helped us with a more unusual fluorescence application.”
“We have used different imagers in the past and they were so complicated to set up that it is great to use the Dyversity system where the software is intuitive. Using the system we have obtained perfect chemiluminescent blot results, without having to stand over them or become a camera expert, which makes Dyversity the perfect imager for our research.”
“In 2007 we started using the Syngene Dyversity system to speed up the imaging of our DIGE* gels. We label our proteins with fluorescent dyes to detect differences in the protein composition and use Coomassie Blue stained gels to identify specific proteins by mass spectrometry. We utilise the Dyversity system, because it offers the possibility to use several wavelengths to detect fluorescent emissions of three or more labeled protein mixtures separated on the same two-dimensional electrophoresis gel.”
“Using the Dyversity system, we have analysed DIGE gels with at least three different colours to produce an image in just two minutes. We have been very happy with the Dyversity system because it has enabled us to rapidly analyse our gels and locate some very interesting proteins associated with neurodegenerative diseases.”
*DIGE is differential 2D gel electrophoresis
“We installed a Dyversity imaging system in 2008 because we needed to accurately detect small amounts of proteins associated with the human aging process. The Dyversity has been very easy to use as the training and the technical support we have had from Syngene have been exceptional.”
“With the system, we have been able to rapidly generate reproducible data which let us measure phosphorylation levels of different signal transduction molecules in insulin-like-growth factor1 pathway so the quantification and analysis capabilities of the Dyversity are exactly right for our work.”
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